Antigen

Antigen Class

TFs and others

Antigen

GLIS3

Cell type

Cell type Class

Pancreas

Cell type

Pancreatic islets

NA

NA

Sample

source_name

pancreatic islet

cell type

Donor derived human islets

chip antibody

GLIS3-antibody pull down

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Human islets were fixed with 1% formalydehyde for 10 minutes at RT, sonicated and insoluble material removed. Illumina Trueseq DNA library preparation kit following manufacturers instructions by the Imperial College genomics facility (index: TTCAGC)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

40542077

Reads aligned (%)

73.6

Duplicates removed (%)

78.0

Number of peaks

140 (qval < 1E-05)

hg19

Number of total reads

40542077

Reads aligned (%)

73.0

Duplicates removed (%)

78.5

Number of peaks

91 (qval < 1E-05)