Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GLIS3

Cell type

Cell type Class
Pancreas
Cell type
Pancreatic islets
NA
NA

Attributes by original data submitter

Sample

source_name
pancreatic islet
cell type
Donor derived human islets
chip antibody
GLIS3-antibody pull down

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Human islets were fixed with 1% formalydehyde for 10 minutes at RT, sonicated and insoluble material removed. Illumina Trueseq DNA library preparation kit following manufacturers instructions by the Imperial College genomics facility (index: TTCAGC)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
40542077
Reads aligned (%)
73.6
Duplicates removed (%)
78.0
Number of peaks
140 (qval < 1E-05)

hg19

Number of total reads
40542077
Reads aligned (%)
73.0
Duplicates removed (%)
78.5
Number of peaks
91 (qval < 1E-05)

Base call quality data from DBCLS SRA